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System with AC generator for cell electroporation
Lukoianov, Petr ; Folprecht, Martin (referee) ; Červinka, Dalibor (advisor)
This bachelor thesis is observing a system with AC generator for cell electroporation. The first paragraph keeps an eye on description of methodology of biophysics mechanisms. In the 2nd part several kinds of devices were described. In the last paragraph you can see a non-assembled construction and operating principle of the device. Apart from that the control circuit logic explored and device manual is included.
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High Voltage Pulse Generator for Electroporation of Cells
Puczok, Václav ; Martiš, Jan (referee) ; Červinka, Dalibor (advisor)
The main goal of this thesis is to design control board for the experimental electroporation device and to develop control firmware. The first chapter of this work focuses on the electroporation phenomenon itself. Behaviour of the cell model in external electrical field is described there as well as simulation and overview of how electroporation affects living tissue. It also explains the main requirements for parameters of the electroporation pulses as well as need for ECG synchronization. Furthermore, some remarks are given about novel high frequency electroporation method, which involves use of nanosecond bipolar high voltage pulse bursts. The second chapter briefly introduces commercial electroporation device called Nanoknife, including control part, power part, and it's limits. The third chapter consists of introduction of the novel experimental electroporation device developed at BUT. Power part of this device is discussed as well. Next chapter focuses on design of the control board for this device and also on description of the particular schematic parts. There is a control algorithm explanation in the fifth chapter of this thesis followed by the brief manual to machine operation.
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System with AC generator for cell electroporation
Lukoianov, Petr ; Folprecht, Martin (referee) ; Červinka, Dalibor (advisor)
This bachelor thesis is observing a system with AC generator for cell electroporation. The first paragraph keeps an eye on description of methodology of biophysics mechanisms. In the 2nd part several kinds of devices were described. In the last paragraph you can see a non-assembled construction and operating principle of the device. Apart from that the control circuit logic explored and device manual is included.
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High Voltage Pulse Generator for Electroporation of Cells
Puczok, Václav ; Martiš, Jan (referee) ; Červinka, Dalibor (advisor)
The main goal of this thesis is to design control board for the experimental electroporation device and to develop control firmware. The first chapter of this work focuses on the electroporation phenomenon itself. Behaviour of the cell model in external electrical field is described there as well as simulation and overview of how electroporation affects living tissue. It also explains the main requirements for parameters of the electroporation pulses as well as need for ECG synchronization. Furthermore, some remarks are given about novel high frequency electroporation method, which involves use of nanosecond bipolar high voltage pulse bursts. The second chapter briefly introduces commercial electroporation device called Nanoknife, including control part, power part, and it's limits. The third chapter consists of introduction of the novel experimental electroporation device developed at BUT. Power part of this device is discussed as well. Next chapter focuses on design of the control board for this device and also on description of the particular schematic parts. There is a control algorithm explanation in the fifth chapter of this thesis followed by the brief manual to machine operation.
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